Pembrolizumab (Keytruda®)药代动力学ELISA试剂盒
货号：EL-1611-161
品牌：AffinityImmuno
产品特性
Pembrolizumab (Keytruda®) Pharmacokinetic ELISA Kit
Reactivity：Human, mouse, rat
Application：Measure free Pembrolizumab
Methode type：Direct sandwich ELISA
Sample type：Serum and Plasma
| 规格 | 价格 |
|---|---|
| 96 wells | ￥咨询 |
| 3 x 96 wells | ￥咨询 |
| 5 x 96 wells | ￥咨询 |
产品咨询：info@biopcr.com
产品订购：sales@biopcr.com
技术支持：tech@biopcr.com
服务热线：400-860-6200
详细介绍
产品名称：Pembrolizumab (Keytruda®)药代动力学ELISA试剂盒
别名：Pembrolizumab Pharmacokinetic ELISA Kit；Pembrolizumab ELISA Kit；Pembrolizumab PK ELISA Kit；Keytruda Pharmacokinetic ELISA Kit；Keytruda ELISA Kit；Pembrolizumab ELISA试剂盒；Pembrolizumab药代动力学ELISA试剂盒；帕博利珠单抗药代动力学ELISA试剂盒；帕博利珠单抗ELISA试剂盒；帕博利珠单抗药代ELISA试剂盒；帕博利珠单抗PK ELISA试剂盒
抗原（Antigen）：Pembrolizumab (Keytruda)
反应性（Reactivity）：Human, mouse, rat
特异性（Specificity）：Pembrolizumab
灵敏度（Sensitivity）：The detection limit is 0.39 ng/mL应用（Application）：Measure free Pembrolizumab
检测类型（Method type）：Direct sandwich ELISA
检测方法（Detection Method）：Peroxidase / OD450
检测精度（Assay precision）：Intra-assay coefficient of variation (CV) <10%. Inter-assay CV was <10%
酶标板类型（Plate）：Strip
样本类型（Sample type）：Serum and Plasma
样本体积（Sample volume）：15ul
防腐剂（Preservative）：None
试剂盒组分（Components）：
Coated microtiter plate, 96 wells
Calibrator diluent. - 1.8ml
Calibrator (500 ug/mL) - 12ul
10X wash buffer - 50ml
Assay buffer - 50ml
1000X detection reagent - 17ul
TMB - 12ml
TMB stop solution - 12ml
Plate sealers - 3
运输条件（Shipping）：Dry ice干冰
储存（Storage）：Stable at -20℃ for 1 year
帕博利珠单抗药代动力学ELISA试剂盒，Pembrolizumab (Keytruda®) Pharmacokinetic ELISA Kit，专用于高特异性、高灵敏度检测游离帕博利珠单抗。该试剂盒适用于可瑞达(Keytruda®)及其生物类似药的检测。
背景信息（Background information）：
Pembrolizumab (Trade name Keytruda®) is a human monoclonal antibody used to block the action of Programmed Cell Death Protein 1(PD-1). Upregulation of PD-1 ligands on tumor cells can allow the tumor to evade anti-tumor immune response. By blocking PDL1 and PD-L2 binding to PD-1, Pembrolizumab induces an anti-tumor immune response by allowing full activation of the anti-tumor T cell response. Thus, Pembrolizumab acts as a checkpoint inhibitor to stimulate immune responses to eliminate cancer cells. The Pembrolizumab ELISA kit is designed to measure free Pembrolizumab with high specificity and sensitivity. The assay design uses a pair of antibodies allowing detection of whole Pembrolizumab molecules in biological matrices.
检测原理（Summary of the assay）：
This assay employs the sandwich enzyme immunoassay technique. Standards and test samples are pipetted into the appropriate wells. Pembrolizumab present in biological matrices is bound by the immobilized capture antibody. After washing away any unbound substances, enzyme linked detection antibody is added to the wells. The plate is washed to remove any unbound antibody enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Pembrolizumab present in test samples.
样本制备（Sample preparation）：
[注意事项]：勃谱生物的帕博利珠单抗PK ELISA试剂盒(Pembrolizumab PK ELISA试剂盒)产品仅用于研究用途。不可用于诊断、医疗或其他用途。
[NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.
返回列表
反应性（Reactivity）：Human, mouse, rat
特异性（Specificity）：Pembrolizumab
灵敏度（Sensitivity）：The detection limit is 0.39 ng/mL应用（Application）：Measure free Pembrolizumab
检测类型（Method type）：Direct sandwich ELISA
检测方法（Detection Method）：Peroxidase / OD450
检测精度（Assay precision）：Intra-assay coefficient of variation (CV) <10%. Inter-assay CV was <10%
酶标板类型（Plate）：Strip
样本类型（Sample type）：Serum and Plasma
样本体积（Sample volume）：15ul
防腐剂（Preservative）：None
试剂盒组分（Components）：
Coated microtiter plate, 96 wells
Calibrator diluent. - 1.8ml
Calibrator (500 ug/mL) - 12ul
10X wash buffer - 50ml
Assay buffer - 50ml
1000X detection reagent - 17ul
TMB - 12ml
TMB stop solution - 12ml
Plate sealers - 3
运输条件（Shipping）：Dry ice干冰
储存（Storage）：Stable at -20℃ for 1 year
帕博利珠单抗药代动力学ELISA试剂盒，Pembrolizumab (Keytruda®) Pharmacokinetic ELISA Kit，专用于高特异性、高灵敏度检测游离帕博利珠单抗。该试剂盒适用于可瑞达(Keytruda®)及其生物类似药的检测。
背景信息（Background information）：
Pembrolizumab (Trade name Keytruda®) is a human monoclonal antibody used to block the action of Programmed Cell Death Protein 1(PD-1). Upregulation of PD-1 ligands on tumor cells can allow the tumor to evade anti-tumor immune response. By blocking PDL1 and PD-L2 binding to PD-1, Pembrolizumab induces an anti-tumor immune response by allowing full activation of the anti-tumor T cell response. Thus, Pembrolizumab acts as a checkpoint inhibitor to stimulate immune responses to eliminate cancer cells. The Pembrolizumab ELISA kit is designed to measure free Pembrolizumab with high specificity and sensitivity. The assay design uses a pair of antibodies allowing detection of whole Pembrolizumab molecules in biological matrices.
检测原理（Summary of the assay）：
This assay employs the sandwich enzyme immunoassay technique. Standards and test samples are pipetted into the appropriate wells. Pembrolizumab present in biological matrices is bound by the immobilized capture antibody. After washing away any unbound substances, enzyme linked detection antibody is added to the wells. The plate is washed to remove any unbound antibody enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Pembrolizumab present in test samples.
样本制备（Sample preparation）：
[注意事项]：勃谱生物的帕博利珠单抗PK ELISA试剂盒(Pembrolizumab PK ELISA试剂盒)产品仅用于研究用途。不可用于诊断、医疗或其他用途。
[NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.
