Filgrastim (G-CSF)药代动力学ELISA试剂盒

抗原（Antigen）：granulocyte colony stimulating factor (G-CSF)
反应性（Reactivity）：human
特异性（Specificity）：GCSF
检测范围（Detection range）：9.375ng/mL - 500 ng/mL
灵敏度（Sensitivity）：9.375 ng/mL应用（Application）：granulocyte colony stimulating factor in biological matrices
检测类型（Method type）：Direct sandwich ELISA
检测方法（Detection Method）：Peroxidase / OD450 
检测精度（Assay precision）：< 25% for ULOQ and LLOQ and <20% for the remaining concentrations
酶标板类型（Plate）：Strip
样本类型（Sample type）：Serum and Plasma
样本体积（Sample volume）：15ul
检测时间（Assay time）：2.5 hours
防腐剂（Preservative）：None
试剂盒组分（Components）：
Coated microtiter plate, 96 wells (1x8 strips) 1 Calibrator diluent 1.8ml Calibrator (1mg/ml) 12μl 20X wash buffer 25ml Assay buffer 50ml 1000X secondary antibody 1000X detection reagent 17μl TMB 12ml TMB stop solution 12ml
运输条件（Shipping）：Dry ice干冰
储存（Storage）：Stable at -20℃ for 1 year

本AffinityImmuno品牌的非格司亭药代动力学ELISA试剂盒，Filgrastim (G-CSF) Pharmacokinetic ELISA Kit，包含检测所需全部组分，适用于定量检测生物基质（如人血清、血浆或细胞培养上清液）中天然及重组人GCSF的含量。

背景信息（Background information）：
Granulocyte colony-stimulating factor (G-CSF or GCSF), also known as colony-stimulating factor 3 (CSF 3), is a glycoprotein that stimulates the bone marrow to produce granulocytes and stem cells and release them into the bloodstream

检测原理（Summary of the assay）：
This assay employs the indirect sandwich enzyme immunoassay technique. Anti-GCSF is coated onto a 96 well microplate. Calibrator and test samples prepared by dilution into assay buffer and are pipetted into the appropriate wells. GCSF present in biological matrices is bound by the immobilized anti- GCSF antibody. After washing away any unbound substances, tagged anti-GCSF secondary antibody is added to the wells. After washing, diluted detection reagent is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of GCSF present in test samples. The color development is stopped and the intensity of the color is measured.

样本采集（Sample collection）：
This kit is compatible with EDTA-plasma, heparinplasma and serum samples. Samples can be stored at or below -20°C for up to 1 year.

样本制备（Sample preparation）：
Dilute calibrators and test samples 1/50 with assay buffer (for example add 5µL of prepared calibrator or sample to 245µL of assay buffer). Mix well. Do not store diluted samples.

试剂准备（Reagent preparation）：
Prepare only the appropriate amount of required reagent on the day of use. Store all reagents as per instructions stated on the label.
1. Wash Buffer (1X) Preparation: Dilute wash buffer concentrate with ultra-pure water 1/20 before use (for example add 25mL concentrate to 475mL ultrapure water). Mix well.
2. Secondary Antibody (1X) Preparation: Dilute secondary antibody with assay buffer 1/1000 before use (for example add 12μl to 12mL of assay buffer). Mix well.
3. Detection Reagent (1X) Preparation: Dilute detection reagent with assay buffer 1/1000 before use (for example add 12μl concentrate to 12ml of assay buffer). Mix well.
4. Calibrator Preparation: Dilute the calibrator from 1mg/ml down to 5µg/ml by pipetting 5µL of calibrator stock into 995µL assay buffer. Label "Cal. Int." Mix well. Prepare calibrators with concentrations ranging from 500 ng/ml to 9.375 ng/ml. The following is an example calibrator curve.

检测程序（Assay procedure）：
This assay employs the indirect sandwich enzyme immunoassay technique. Anti-GCSF is coated onto a 96 well microplate. Calibrator and test samples prepared by dilution into assay buffer and are pipetted into the appropriate wells. GCSF present in biological matrices is bound by the immobilized anti- GCSF antibody. After washing away any unbound substances, tagged anti-GCSF secondary antibody is added to the wells. After washing, diluted detection reagent is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of GCSF present in test samples. The color development is stopped and the intensity of the color is measured.

结果计算（Results calculation）：
1. Construct a standard curve by plotting the absorbance obtained from each standard against concentration. Use a 4 or 5 parameter curve fit.
2. The concentration of the unknowns can be back calculated directly from this standard curve using the absorbance value for each sample.
3. Any sample diluted more or less than the standard series will need additional data correction. For example, if the sample is diluted 1/50, then the concentration will be calculated by dividing by 2 due to the calibrators being 2 times more diluted. Similarly, if the sample is diluted 1/500, then the concentration will be calculated by multiplying by a correction factor of 5 due to the calibrators being 5 times more concentrated.

[注意事项]：勃谱生物的非格司亭PK ELISA试剂盒(G-CSF PK ELISA试剂盒)产品仅用于研究用途。不可用于诊断、医疗或其他用途。
[NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.