GCSF抗药抗体（ADA） ELISA试剂盒
货号：EL-141-73196
品牌：AffinityImmuno
产品特性
Granulocyte Colony-Stimulating Factor(GCSF) ADA ELISA Kit
Reactivity：human
Application：Quantification of antibodies to G-CSF
Methode type：Direct sandwich ELISA
Sample type：Serum and Plasma
| 规格 | 价格 |
|---|---|
| 96 wells | ￥咨询 |
| 3 x 96 wells | ￥咨询 |
| 5 x 96 wells | ￥咨询 |
产品咨询：info@biopcr.com
产品订购：sales@biopcr.com
技术支持：tech@biopcr.com
服务热线：400-860-6200
详细介绍
产品名称：GCSF抗药抗体（ADA） ELISA试剂盒
别名：GCSF ADA ELISA Kit；granulocyte colony stimulating factor ELISA Kit；G-CSF Anti-Drug Antibody ELISA Kit；GCSF ELISA试剂盒；G-CSF抗药物抗体检测试剂盒；GCSF ADA ELISA试剂盒；G-CSF抗药物抗体检测试剂盒;GCSF抗药抗体ELISA试剂盒；粒细胞集落刺激因子ADA ELISA试剂盒；粒细胞集落刺激因子ELISA试剂盒；粒细胞集落刺激因子抗药抗体ELISA试剂盒
抗原（Antigen）：antibodies to GCSF
反应性（Reactivity）：human
特异性（Specificity）：anti-GCSF antibodies
检测范围（Detection range）：45 ng/mL -1440 ng/mL
灵敏度（Sensitivity）：45 ng/mL应用（Application）：Quantification of antibodies to G-CSF
检测类型（Method type）：Direct sandwich ELISA
检测方法（Detection Method）：Peroxidase / OD450
检测精度（Assay precision）：<20%, <20%
酶标板类型（Plate）：Strip
样本类型（Sample type）：Serum and Plasma
样本体积（Sample volume）：15ul
检测时间（Assay time）：2.5 hours
防腐剂（Preservative）：none
试剂盒组分（Components）：
Coated microtiter plate, 96 wells (12x8 strips) 1 Calibrator diluent 1.8 ml Calibrator (1.15mg/mL) 12 μl 10X wash buffer 50 ml Assay buffer 50 ml 1000X detection reagent 17 μl TMB 12 ml TMB stop solution
运输条件（Shipping）：Wet ice湿冰
储存（Storage）：Stable at -20℃ for 1 year
GCSF抗药抗体ELISA试剂盒，Granulocyte Colony-Stimulating Factor(GCSF) ADA ELISA Kit，本AffinityImmuno G-CSF抗药抗体ELISA试剂盒包含检测生物基质，如人血清、血浆或细胞培养上清液中抗GCSF抗体所需的全套组分。
背景信息（Background information）：
Granulocyte colony-stimulating factor (G-CSF or GCSF), also known as colony-stimulating factor 3 (CSF 3), is a glycoprotein that stimulates the bone marrow to produce granulocytes and stem cells and release them into the bloodstream
检测原理（Summary of the assay）：
This assay employs the indirect enzyme immunoassay technique. GCSF is coated onto a 96 well microplate. Calibrator and test samples are prepared by dilution into assay buffer and are pipetted into the appropriate wells. Antibodies to GCSF present in biological matrices are bound to the immobilized GCSF. After washing away any unbound substances, antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of antibodies to GCSF present in the calibrator and test samples. The color development is stopped and the intensity of the color is measured.
样本采集（Sample collection）：
This kit is compatible with EDTA-plasma, heparinplasma and serum samples. Samples can be stored at or below -20°C for up to 1 year.
样本制备（Sample preparation）：
Dilute calibrators and test samples 1/50 with assay buffer (for example add 5µl of prepared calibrator or sample to 245µl of assay buffer). Mix well. Do not store diluted samples.
试剂准备（Reagent preparation）：
Prepare only the appropriate amount of required reagent on the day of use. Store all reagents as per instructions stated on the label.
1. Wash Buffer (1X) Preparation:Dilute wash buffer concentrate with ultra-pure water 1/20 before use (for example, add 50mL wash buffer concentrate to 450mL ultra-pure water). Mix well.
2. Detection Reagent (1X) Preparation: Dilute detection reagent with assay buffer 1/1000 before use (for example add 11μl concentrate to 11ml of assay buffer). Mix well.
3. Calibrator Preparation: Dilute the calibrator from 1.15mg/ml down to 11.5µg/ml by pipetting 5µl of calibrator stock into 495µl assay buffer. Label "Cal. Int." Mix well. Prepare calibrators with concentrations ranging from 1440ng/ml to 45ng/ml. The following is an example calibrator curve
检测程序（Assay procedure）：
This assay employs the indirect enzyme immunoassay technique. GCSF is coated onto a 96 well microplate. Calibrator and test samples are prepared by dilution into assay buffer and are pipetted into the appropriate wells. Antibodies to GCSF present in biological matrices are bound to the immobilized GCSF. After washing away any unbound substances, antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of antibodies to GCSF present in the calibrator and test samples. The color development is stopped and the intensity of the color is measured
结果计算（Results calculation）：
1. Construct a standard curve by plotting the absorbance obtained from each standard against concentration. Use a 4 or 5 parameter curve fit.
2. The concentration of the unknowns can be back calculated directly from this standard curve using the absorbance value for each sample.
[注意事项]：勃谱生物的GCSF抗药抗体ELISA试剂盒(GCSF ADA ELISA试剂盒)产品仅用于研究用途。不可用于诊断、医疗或其他用途。
[NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.
返回列表
反应性（Reactivity）：human
特异性（Specificity）：anti-GCSF antibodies
检测范围（Detection range）：45 ng/mL -1440 ng/mL
灵敏度（Sensitivity）：45 ng/mL应用（Application）：Quantification of antibodies to G-CSF
检测类型（Method type）：Direct sandwich ELISA
检测方法（Detection Method）：Peroxidase / OD450
检测精度（Assay precision）：<20%, <20%
酶标板类型（Plate）：Strip
样本类型（Sample type）：Serum and Plasma
样本体积（Sample volume）：15ul
检测时间（Assay time）：2.5 hours
防腐剂（Preservative）：none
试剂盒组分（Components）：
Coated microtiter plate, 96 wells (12x8 strips) 1 Calibrator diluent 1.8 ml Calibrator (1.15mg/mL) 12 μl 10X wash buffer 50 ml Assay buffer 50 ml 1000X detection reagent 17 μl TMB 12 ml TMB stop solution
运输条件（Shipping）：Wet ice湿冰
储存（Storage）：Stable at -20℃ for 1 year
GCSF抗药抗体ELISA试剂盒，Granulocyte Colony-Stimulating Factor(GCSF) ADA ELISA Kit，本AffinityImmuno G-CSF抗药抗体ELISA试剂盒包含检测生物基质，如人血清、血浆或细胞培养上清液中抗GCSF抗体所需的全套组分。
背景信息（Background information）：
Granulocyte colony-stimulating factor (G-CSF or GCSF), also known as colony-stimulating factor 3 (CSF 3), is a glycoprotein that stimulates the bone marrow to produce granulocytes and stem cells and release them into the bloodstream
检测原理（Summary of the assay）：
This assay employs the indirect enzyme immunoassay technique. GCSF is coated onto a 96 well microplate. Calibrator and test samples are prepared by dilution into assay buffer and are pipetted into the appropriate wells. Antibodies to GCSF present in biological matrices are bound to the immobilized GCSF. After washing away any unbound substances, antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of antibodies to GCSF present in the calibrator and test samples. The color development is stopped and the intensity of the color is measured.
样本采集（Sample collection）：
This kit is compatible with EDTA-plasma, heparinplasma and serum samples. Samples can be stored at or below -20°C for up to 1 year.
样本制备（Sample preparation）：
Dilute calibrators and test samples 1/50 with assay buffer (for example add 5µl of prepared calibrator or sample to 245µl of assay buffer). Mix well. Do not store diluted samples.
试剂准备（Reagent preparation）：
Prepare only the appropriate amount of required reagent on the day of use. Store all reagents as per instructions stated on the label.
1. Wash Buffer (1X) Preparation:Dilute wash buffer concentrate with ultra-pure water 1/20 before use (for example, add 50mL wash buffer concentrate to 450mL ultra-pure water). Mix well.
2. Detection Reagent (1X) Preparation: Dilute detection reagent with assay buffer 1/1000 before use (for example add 11μl concentrate to 11ml of assay buffer). Mix well.
3. Calibrator Preparation: Dilute the calibrator from 1.15mg/ml down to 11.5µg/ml by pipetting 5µl of calibrator stock into 495µl assay buffer. Label "Cal. Int." Mix well. Prepare calibrators with concentrations ranging from 1440ng/ml to 45ng/ml. The following is an example calibrator curve
检测程序（Assay procedure）：
This assay employs the indirect enzyme immunoassay technique. GCSF is coated onto a 96 well microplate. Calibrator and test samples are prepared by dilution into assay buffer and are pipetted into the appropriate wells. Antibodies to GCSF present in biological matrices are bound to the immobilized GCSF. After washing away any unbound substances, antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of antibodies to GCSF present in the calibrator and test samples. The color development is stopped and the intensity of the color is measured
结果计算（Results calculation）：
1. Construct a standard curve by plotting the absorbance obtained from each standard against concentration. Use a 4 or 5 parameter curve fit.
2. The concentration of the unknowns can be back calculated directly from this standard curve using the absorbance value for each sample.
[注意事项]：勃谱生物的GCSF抗药抗体ELISA试剂盒(GCSF ADA ELISA试剂盒)产品仅用于研究用途。不可用于诊断、医疗或其他用途。
[NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.
