抗人Defensin-5(8C8)抗体-超低内毒素
货号：ICH1164UL-5mg
品牌：ichorbio
产品特性
Bulk Anti-Human Defensin-5 (8C8) Antibody - Ultra-Low Endotoxin is manufactured in an cGMP compliant facility.
内毒素: ≤ 0.5 EU/mg as determined by the LAL method
纯度: >98% by SDS-PAGE and HPLC
应用: Dot Blot, ELISA, IHC (Paraffin), Western Blot.
| 规格 | 价格 |
|---|---|
| 5mg | ￥咨询 |
产品咨询：info@biopcr.com
产品订购：sales@biopcr.com
技术支持：tech@biopcr.com
服务热线：400-860-6200
详细介绍
产品名称：抗人Defensin-5(8C8)抗体-超低内毒素
别名：抗人Defensin-5抗体, Bulk, Anti-Human Defensin-5 (8C8) Antibody - Ultra-Low Endotoxin, Anti-Human Defensin-5 Antibody, DEFA-5, defensin, alpha 5, HD10
宿主：Mouse
应用：Dot Blot, ELISA, IHC (Paraffin), Western Blot
亚型：Mouse IgG2b Kappa
克隆：8C8
浓度：1.0 – 5.0 mg/ml
免疫原：Recombinant Human HD5 (aa 20-94).
反应性：Human
特异性：Clone 8C8 recognizes human defensin 5
存储溶液：This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and azide free
储存条件：This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
推荐同型对照（Isotype Control）：Mouse IgG2b Isotype Control for In Vivo - Low Endotoxin [MPC-11] (ICH2250)
推荐缓冲液（Buffer）：体内抗体缓冲液pH5.0； 体内抗体缓冲液pH7.4； 体内抗体缓冲液pH8.0
靶点（Target）: Defensin-5
UniProt：Q01523
纯化方法（Purification method）: This monoclonal antibody was purified using Protein A
抗原分布（Antigen Distribution）：HD5 is highly expressed in the secretory granules of Paneth cells of the ileum.
背景资料（Background）：The colon lies adjacent to the small intestine and has heavy bacterial colonization from ingested food and water that is often contaminated with bacteria. Remarkably, the small intestine has a low microbial density. A special type of epithelial cell, called a Paneth cell, can be found clustered at the base of the tubular glands that lie between the villi of the inner surface of the small intestine. These cells secrete defensins which have been shown to have activity against both Gram-positive and Gram-negative microbes.3 Six human α-defensins, a subfamily of defensin peptides characterized by their cysteine spacing and disulfide connectivity2, have been identified. Human Paneth cells express α-defensin 5 (HD5) along with HD6. Paneth cells are most numerous in the ileum and have many features similar to those of myeloid cells. They are multifaceted cells with a large quantity of apically-located eosinophilic secretory granules containing lysozyme and other antimicrobial factors which are released upon bacterial stimulation.2 In addition, these cells express tumor necrosis factor alpha (TNF-a), CD1, and CD15.1 Paneth cells do not store defensins as fully processed or active peptides, rather they store them as inactive propeptides.2 They are released as mature peptides after trypsin digestion. Trypsin is also secreted from the Paneth cell granules. It has been reported that disrupted α-defensin processing in murine Paneth cells has initiated a vulnerability to enteric infection.2 HD5 expression levels have been found to be negatively correlated to intestinal infection. Additionally, studies have shown HD5 to be a strong antagonist towards human Papillomavirus infection. Furthermore, low expression of HD5 is thought to play a role in Crohn’s disease.3 Anti-Human Alpha Defensin-5 (HD5) Clone 8C8 has been shown to recognize the propeptide and the partially processed forms of HD5. However, clone 8C8 has been reported to scarcely recognize the mature peptide. There was no reported cross reactivity with HD6, lysozyme, or sPLA2.
纯度（Purity）：>98% by SDS-PAGE and HPLC
内毒素（Endotoxin）：≤ 0.5 EU/mg as determined by the LAL method
聚集（Aggregation）：Aggregation level ≤ 1%
IMPACT病原体检测（IMPACT Pathogen Test）：We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our mouse antibodies are tested for: Mycoplasma spp.
Mycoplasma pulmonis
Sendai virus
Mouse hepatitis virus
Pneumonia virus of mice
Minute virus of mice
Mouse parvovirus (MPV1-5)
Theiler’s murine encephalomyelitis virus
Murine norovirus
Reovirus 3
Mouse rotavirus
Ectromelia virus
Lymphocytic choriomeningitis virus
Polyoma virus
Lactate dehydrogenase-elevating virus
Mouse adenovirus (MAD1, MAD2)
Mouse cytomegalovirus
K virus
Mouse thymic virus
Hantaan virus
Corynebacterium bovis
Corynebacterium spp. (HAC2)
应用说明（Application Notes）：Each investigator should determine their own optimal working dilution for specific applications.
产品使用（Use）：Products are for research use only. Not for use in diagnostic or therapeutic procedures.
运输条件：Blue ice
返回列表
推荐缓冲液（Buffer）：体内抗体缓冲液pH5.0； 体内抗体缓冲液pH7.4； 体内抗体缓冲液pH8.0
靶点（Target）: Defensin-5
UniProt：Q01523
纯化方法（Purification method）: This monoclonal antibody was purified using Protein A
抗原分布（Antigen Distribution）：HD5 is highly expressed in the secretory granules of Paneth cells of the ileum.
背景资料（Background）：The colon lies adjacent to the small intestine and has heavy bacterial colonization from ingested food and water that is often contaminated with bacteria. Remarkably, the small intestine has a low microbial density. A special type of epithelial cell, called a Paneth cell, can be found clustered at the base of the tubular glands that lie between the villi of the inner surface of the small intestine. These cells secrete defensins which have been shown to have activity against both Gram-positive and Gram-negative microbes.3 Six human α-defensins, a subfamily of defensin peptides characterized by their cysteine spacing and disulfide connectivity2, have been identified. Human Paneth cells express α-defensin 5 (HD5) along with HD6. Paneth cells are most numerous in the ileum and have many features similar to those of myeloid cells. They are multifaceted cells with a large quantity of apically-located eosinophilic secretory granules containing lysozyme and other antimicrobial factors which are released upon bacterial stimulation.2 In addition, these cells express tumor necrosis factor alpha (TNF-a), CD1, and CD15.1 Paneth cells do not store defensins as fully processed or active peptides, rather they store them as inactive propeptides.2 They are released as mature peptides after trypsin digestion. Trypsin is also secreted from the Paneth cell granules. It has been reported that disrupted α-defensin processing in murine Paneth cells has initiated a vulnerability to enteric infection.2 HD5 expression levels have been found to be negatively correlated to intestinal infection. Additionally, studies have shown HD5 to be a strong antagonist towards human Papillomavirus infection. Furthermore, low expression of HD5 is thought to play a role in Crohn’s disease.3 Anti-Human Alpha Defensin-5 (HD5) Clone 8C8 has been shown to recognize the propeptide and the partially processed forms of HD5. However, clone 8C8 has been reported to scarcely recognize the mature peptide. There was no reported cross reactivity with HD6, lysozyme, or sPLA2.
纯度（Purity）：>98% by SDS-PAGE and HPLC
内毒素（Endotoxin）：≤ 0.5 EU/mg as determined by the LAL method
聚集（Aggregation）：Aggregation level ≤ 1%
IMPACT病原体检测（IMPACT Pathogen Test）：We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our mouse antibodies are tested for: Mycoplasma spp.
Mycoplasma pulmonis
Sendai virus
Mouse hepatitis virus
Pneumonia virus of mice
Minute virus of mice
Mouse parvovirus (MPV1-5)
Theiler’s murine encephalomyelitis virus
Murine norovirus
Reovirus 3
Mouse rotavirus
Ectromelia virus
Lymphocytic choriomeningitis virus
Polyoma virus
Lactate dehydrogenase-elevating virus
Mouse adenovirus (MAD1, MAD2)
Mouse cytomegalovirus
K virus
Mouse thymic virus
Hantaan virus
Corynebacterium bovis
Corynebacterium spp. (HAC2)
应用说明（Application Notes）：Each investigator should determine their own optimal working dilution for specific applications.
产品使用（Use）：Products are for research use only. Not for use in diagnostic or therapeutic procedures.
运输条件：Blue ice
