产品特性
5-HT (Serotonin) 2C Receptor Antibody
应用：ICC,IF,IHC,WB验证
Host:Rabbit；Immunogen：Rat 5-HT2C receptor (439-460)
高文献引用数据支持（通过CiteAb官网可查）
| 规格 | 价格 |
|---|---|
| 100 µL | ￥咨询 |
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详细介绍
产品名称：5-HT (Serotonin) 2C受体抗体
别名：5-HT2C受体抗体；5-羟色胺2C受体抗体；5-HT2C Receptor Antibody；5-hydroxytryptamine receptor 2C; 5-HT2C; 5HT-1C; 5-HTR2C; 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled, anti-5-HT 2C
宿主：Rabbit
应用：ICC,IF,IHC,WB验证
免疫原：Rat 5-HT2C receptor (439-460)
反应性：Mouse, Rat
形式：液体
描述：
The ImmunoStar 5-HT2C receptor antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat choroid plexus and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/500 – 1/1000 in PBS – Bn/Av-HRP technique. Intensification methods such as nickel will approximately double the dilution factor as recommended.
The antibody was characterized by immunohistochemistry and Western blot. Western blotting revealed a single band of approximately 70 kD. Due to the difficulty with receptor antibodies, western blot applications are not warranted and are included as specificity information only.
Preincubation of the antibody with an excess of the synthetic peptide blocked staining. Immunohistochemical staining of rat brain correlates well with Northern analysis, in situ hybridization and receptor autoradiography. BlastP database sequence homology searches confirmed that this sequence is unique to rat, mouse and human 5-HT2C receptors.
图片说明：
Gray scale IHC image of coronal rat brain section staining for the 5-HT2C receptor (above) and high magnification image of receptor expression in the cortex of the rat brain (below). The tissue was fixed with 4% formaldehyde/0.05% glutaraldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:1000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
RRID：AB_572212
GeneSymbol：Htr2c
运输条件：Blue ice
返回列表
The ImmunoStar 5-HT2C receptor antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat choroid plexus and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/500 – 1/1000 in PBS – Bn/Av-HRP technique. Intensification methods such as nickel will approximately double the dilution factor as recommended.
The antibody was characterized by immunohistochemistry and Western blot. Western blotting revealed a single band of approximately 70 kD. Due to the difficulty with receptor antibodies, western blot applications are not warranted and are included as specificity information only.
Preincubation of the antibody with an excess of the synthetic peptide blocked staining. Immunohistochemical staining of rat brain correlates well with Northern analysis, in situ hybridization and receptor autoradiography. BlastP database sequence homology searches confirmed that this sequence is unique to rat, mouse and human 5-HT2C receptors.
图片说明：
Gray scale IHC image of coronal rat brain section staining for the 5-HT2C receptor (above) and high magnification image of receptor expression in the cortex of the rat brain (below). The tissue was fixed with 4% formaldehyde/0.05% glutaraldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:1000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
RRID：AB_572212
GeneSymbol：Htr2c
运输条件：Blue ice
