产品特性
VAT (Vesicular Acetylcholine Transporter) Antibody
应用:ICC,IF,IHC,WB验证
Host:Goat
高文献引用数据支持(通过CiteAb官网可查)
规格 | 价格 |
---|---|
100 µL | ¥咨询 |
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详细介绍
产品名称:VAT (Vesicular Acetylcholine Transporter)抗体
别名:Solute carrier family 18, member 3; solute carrier family 18 (vesicular monoamine) member 3; solute carrier family 18 (vesicular acetylcholine), member 3; rVAT; VACht, anti-VAT, anti-VACht;囊泡乙酰胆碱转运体抗体
宿主:Goat
应用:ICC,IF,IHC,WB验证
反应性:Human, Mouse, Rat
形式:Lyophilized Whole Serum
描述:
The antibody produces strong labeling of VAChT at a dilution of 1/3,000 – 1/5,000 using biotin-streptavidin/HRP technique in rat basal forebrain. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended.
Immunolabeling is completely abolished by pre-adsorption with synthetic rat VAChT (511-530). Immunolabeling of transfected cells demonstrates no cross reactivity with vesicular monoamine transporters.
图片说明:
IHC image of neurons in the rat basal forebrain staining for VAT. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% rabbit serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, rabbit anti-goat secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
GeneSymbol:Slc18a3
运输条件:Blue ice
返回列表
The antibody produces strong labeling of VAChT at a dilution of 1/3,000 – 1/5,000 using biotin-streptavidin/HRP technique in rat basal forebrain. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended.
Immunolabeling is completely abolished by pre-adsorption with synthetic rat VAChT (511-530). Immunolabeling of transfected cells demonstrates no cross reactivity with vesicular monoamine transporters.
图片说明:
IHC image of neurons in the rat basal forebrain staining for VAT. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% rabbit serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, rabbit anti-goat secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
GeneSymbol:Slc18a3
运输条件:Blue ice