产品特性
Beta-Endorphin Antibody
应用:ICC,IF,IHC,WB验证
Host:Rabbit;Immunogen:Beta-Endorphin
高文献引用数据支持(通过CiteAb官网可查)
规格 | 价格 |
---|---|
100 µL | ¥咨询 |
产品咨询:info@biopcr.com
产品订购:sales@biopcr.com
技术支持:tech@biopcr.com
服务热线:400-860-6200
详细介绍
产品名称:Beta-Endorphin抗体
别名:Beta-内啡肽抗体;Endorfin-beta; Endorphin-beta; proopiomelanocortin, anti-Beta-Endorphin
宿主:Rabbit
应用:ICC,IF,IHC,WB验证
免疫原:Beta-Endorphin
反应性:Carp, Cat, Guinea Pig, Human, Mouse, Newt, Rat
形式:Lyophilized
描述:
The antibody produces a strong biotin-streptavidin/HRP staining at a 1/1000 – 1/2000 dilution in rat anterior pituitary.
Staining is completely eliminated by pretreatment of the diluted antibody with 10-6 M of Beta-Endorphin. Pre-adsorption of the diluted antibody with 10-6M of the following substances had no effect on Beta-Endorphin labeling: methionine enkephalin, leucine enkaphalin, dynorphin A, dynorphin B, gamma-endorphin, alpha-endorphin, ACTH and alpha-melanocyte stimulating hormone.
图片说明:
IHC images of beta-endorphin staining in the rat anterior pituitary with FITC-conjugated secondary antibody (above) and with standard biotin-streptavidin/HRP and DAB chromogen (below). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for frozen sectioning. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:2000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS.
Preabsorption Control: Available at Sigma Aldrich, Catalog # E1142
RRID:AB_572220
GeneSymbol:POMC
运输条件:Blue ice
返回列表
The antibody produces a strong biotin-streptavidin/HRP staining at a 1/1000 – 1/2000 dilution in rat anterior pituitary.
Staining is completely eliminated by pretreatment of the diluted antibody with 10-6 M of Beta-Endorphin. Pre-adsorption of the diluted antibody with 10-6M of the following substances had no effect on Beta-Endorphin labeling: methionine enkephalin, leucine enkaphalin, dynorphin A, dynorphin B, gamma-endorphin, alpha-endorphin, ACTH and alpha-melanocyte stimulating hormone.
图片说明:
IHC images of beta-endorphin staining in the rat anterior pituitary with FITC-conjugated secondary antibody (above) and with standard biotin-streptavidin/HRP and DAB chromogen (below). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for frozen sectioning. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:2000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS.
Preabsorption Control: Available at Sigma Aldrich, Catalog # E1142
RRID:AB_572220
GeneSymbol:POMC
运输条件:Blue ice