产品特性
Insulin Antibody
应用:ICC,IF,IHC,WB验证
Host:Rabbit
高文献引用数据支持(通过CiteAb官网可查)
规格 | 价格 |
---|---|
100 µL | ¥咨询 |
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详细介绍
产品名称:Insulin抗体
别名:anti-Insulin; INS;兔胰岛素抗体
宿主:Rabbit
应用:ICC,IF,IHC,WB验证
反应性:Bubalus Bubalis (Buffalo), Cattle, Chick, Cow, Heifer (Cow), Horse, Human, Lizard, Macaque, Manducta Sexta (Moth), Monkey, Mouse, Pig, Rat, Sheep
形式:Lyophilized Whole Serum
描述:
The ImmunoStar Insulin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat pancreatic beta cells using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/3000-1/5000 in PBS/0.3% Triton X-100 – biotin/avidin-HRP Technique.
Immuostaining is completely abolished by soluble pre-adsorption with bovine insulin at 10 µg per mL of diluted antiserum.
图片说明:
IHC image of beta cells in the islets of Langerhans of the rat pancreas staining for insulin (above) and counterstained with hemotoxylin (below). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for frozen sectioning. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen
GeneSymbol:INS
运输条件:Blue ice
返回列表
The ImmunoStar Insulin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat pancreatic beta cells using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/3000-1/5000 in PBS/0.3% Triton X-100 – biotin/avidin-HRP Technique.
Immuostaining is completely abolished by soluble pre-adsorption with bovine insulin at 10 µg per mL of diluted antiserum.
图片说明:
IHC image of beta cells in the islets of Langerhans of the rat pancreas staining for insulin (above) and counterstained with hemotoxylin (below). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for frozen sectioning. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen
GeneSymbol:INS
运输条件:Blue ice