产品特性
nNOS:N-Terminal (neuronal Nitric Oxide Synthase) Antibody
应用：ICC,IF,IHC,WB验证
Host:Rabbit
高文献引用数据支持（通过CiteAb官网可查）
| 规格 | 价格 |
|---|---|
| 100 µL | ￥咨询 |
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详细介绍
产品名称：nNOS:N-Terminal (neuronal Nitric Oxide Synthase)抗体
别名：Constitutive NOS; NC-NOS; NOS type I; Neuronal NOS; Nitric Oxide Synthase; bNOS; Nitric oxide synthase-brain; N-NOS; Peptidyl-cysteine S-nitrosylase; IHPS1; NC-NOS; nitric oxide synthase 1 (neuronal), anti-nNOS:N-Terminal Antibody
宿主：Rabbit
应用：ICC,IF,IHC,WB验证
反应性： Mouse, Rat
形式：Lyophilized Whole Serum
描述：
The ImmunoStar N-terminal neuronal nitric oxide synthase antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus, striatum, cortex and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/1,000 – 1/2,000 in PBS/0.3% Triton X-100 – biotin/avidin-HRP.
By Western blot analysis of brain homogenates the antibody specifically labels a band of approximately 155 kD. Immunolabeling is completely abolished by pre-adsorption with synthetic human nNOS (134-148) at 5 µg per mL of diluted antibody. No cross reactivity with other forms of NOS was observed.
图片说明：
IHC image of neurons staining for the N-terminal of nNOS in the rat cortex (above and below left) and the rat striatum (below right). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:2000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen, bottom left image with nickel stain.
GeneSymbol/ID,Accession#:NOS1,4842
运输条件：Blue ice
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The ImmunoStar N-terminal neuronal nitric oxide synthase antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus, striatum, cortex and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/1,000 – 1/2,000 in PBS/0.3% Triton X-100 – biotin/avidin-HRP.
By Western blot analysis of brain homogenates the antibody specifically labels a band of approximately 155 kD. Immunolabeling is completely abolished by pre-adsorption with synthetic human nNOS (134-148) at 5 µg per mL of diluted antibody. No cross reactivity with other forms of NOS was observed.
图片说明：
IHC image of neurons staining for the N-terminal of nNOS in the rat cortex (above and below left) and the rat striatum (below right). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:2000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen, bottom left image with nickel stain.
GeneSymbol/ID,Accession#:NOS1,4842
运输条件：Blue ice
