产品特性
Assay principle: Competitive ELISA (detection: 450 nm)
Measuring range: 2 - 700 nmol/L
Specific to N-epsilon-Hexanoyl-Lysine adduct
Suitable for assessment of oxidative stress using urine, serum and cultured cells.
| 规格 | 价格 |
|---|---|
| 96 wells | ￥咨询 |
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详细介绍
产品名称：Hexanoyl-Lysine adduct(HEL) ELISA试剂盒
别名：Hexanoyl-Lysine adduct ELISA Kit；Hexanoyl-Lys ELISA Kit；Hexanoyl-Lysine ELISA Kit；HEL ELISA Kit；Hexanoyl-Lysine adduct ELISA试剂盒；Hexanoyl-Lys ELISA试剂盒；Hexanoyl-Lysine ELISA试剂盒；HEL ELISA试剂盒；己酰-赖氨酸加合物ELISA试剂盒；己酰赖氨酸加合物ELISA试剂盒；己酰-赖氨酸ELISA试剂盒；己酰赖氨酸ELISA试剂盒；己酰赖氨酸检测试剂盒
应用：Urine, serum and cultured cells from human and animals.
特异性：Specific to N-epsilon-Hexanoyl-Lysine adduct
检测方法：Competitive ELISA (detection: 450 nm)
检测范围：2 - 700 nmol/L
形式：96 wells (54 samples in single assay)
储存条件：储存于2 - 8°C（避免冷冻）
关于己酰-赖氨酸（HEXANOYL-LYSINE, HEL）
早期脂质氧化生物标志物
Oxidative damage of lipids caused by reactive oxygen species (ROS) play an important role in some diseases, lesion of cell functions and aging. Aldehydes such as malondi-aldehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxyoctadecanoic acid (13-HPODE) is found to be covalently bound to proteins1). Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine residues. HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid. HEL may be a useful biomarker for initial stage of lipid peroxidation.Monoclonal antibodies and ELISA kit have been developped, and HEL can be detected in oxidatively modified LDL, in human atherosclerotic lesions, human urine and serum. It is also reported that HEL is formed in rat muscle during exercise, and the formation is prohibited by antioxidants such as flavonoids.
Hexanoyl-Lys (HEL) ELISA检测试剂盒
JaICA have developed HEL ELISA kit in collaboration with Dr. Toshihiko Osawa (Nagoya University) and Dr. Yoji Kato (Univerisity of Hyogo). This ELISA kit can be applied to urine, serum and cultured cells form human and animal.
本试剂盒适用于人及动物的尿液、血清和培养细胞样本的检测。
试剂盒组分
HEL-coated Microtiter Plate: 1 plate (96 wells)
Primary Antibody (ready to use): 1 vial
Secondary Antibody: 1 vial
Secondary Antibody Buffer: 1 vial
Chromogen (TMBZ solution): 1 vial
Chromogen Buffer: 1 vial
Washing Buffer (5X): 1 vial
Stop Solution: 1 vial
Standard solution (6 levels): 1 vial each
Plate seal: 2 sheets
需用户自备
50 µL micropipettor and pipette tips
8-channel (50-200 µL) micropipettor and tips
8 or 12-syncronous multichannel pipet and reagent tray for multichannel pipet.
4-7 °C incubator
Microtiter plate reader (measuring wavelength 450 nm)
[注意事项]：该己酰-赖氨酸加合物ELISA试剂盒仅用于研究用途。不可用于诊断、医疗或其他用途。
[NOTICE]: Our Hexanoyl-Lys adduct (HEL) ELISA kit are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.
返回列表
早期脂质氧化生物标志物
Oxidative damage of lipids caused by reactive oxygen species (ROS) play an important role in some diseases, lesion of cell functions and aging. Aldehydes such as malondi-aldehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxyoctadecanoic acid (13-HPODE) is found to be covalently bound to proteins1). Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine residues. HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid. HEL may be a useful biomarker for initial stage of lipid peroxidation.Monoclonal antibodies and ELISA kit have been developped, and HEL can be detected in oxidatively modified LDL, in human atherosclerotic lesions, human urine and serum. It is also reported that HEL is formed in rat muscle during exercise, and the formation is prohibited by antioxidants such as flavonoids.
Hexanoyl-Lys (HEL) ELISA检测试剂盒
JaICA have developed HEL ELISA kit in collaboration with Dr. Toshihiko Osawa (Nagoya University) and Dr. Yoji Kato (Univerisity of Hyogo). This ELISA kit can be applied to urine, serum and cultured cells form human and animal.
本试剂盒适用于人及动物的尿液、血清和培养细胞样本的检测。
试剂盒组分
HEL-coated Microtiter Plate: 1 plate (96 wells)
Primary Antibody (ready to use): 1 vial
Secondary Antibody: 1 vial
Secondary Antibody Buffer: 1 vial
Chromogen (TMBZ solution): 1 vial
Chromogen Buffer: 1 vial
Washing Buffer (5X): 1 vial
Stop Solution: 1 vial
Standard solution (6 levels): 1 vial each
Plate seal: 2 sheets
需用户自备
50 µL micropipettor and pipette tips
8-channel (50-200 µL) micropipettor and tips
8 or 12-syncronous multichannel pipet and reagent tray for multichannel pipet.
4-7 °C incubator
Microtiter plate reader (measuring wavelength 450 nm)
[注意事项]：该己酰-赖氨酸加合物ELISA试剂盒仅用于研究用途。不可用于诊断、医疗或其他用途。
[NOTICE]: Our Hexanoyl-Lys adduct (HEL) ELISA kit are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.
