Tocilizumab (Actemra®)免疫原性ELISA试剂盒
货号：EL-141-202
品牌：AffinityImmuno
产品特性
Tocilizumab (Actemra®) Immunogenicity ELISA Kit
Detection minimum：detection limit 1.5 ng/mL
Application：measure anti-tocilizumab antibodies
Methode type：Bridging ELISA
Sample type：Serum and Plasma
| 规格 | 价格 |
|---|---|
| 96 wells | ￥咨询 |
| 3 x 96 wells | ￥咨询 |
| 5 x 96 wells | ￥咨询 |
产品咨询：info@biopcr.com
产品订购：sales@biopcr.com
技术支持：tech@biopcr.com
服务热线：400-860-6200
详细介绍
产品名称：Tocilizumab (Actemra®)免疫原性ELISA试剂盒
别名：Tocilizumab Immunogenicity ELISA Kit；Tocilizumab ELISA Kit；Actemra免疫原性ELISA试剂盒；Actemra ELISA试剂盒；Tocilizumab免疫原性ELISA试剂盒；Tocilizumab ELISA试剂盒；托珠单抗免疫原性ELISA试剂盒；托珠单抗ELISA试剂盒
抗原（Antigen）：Tocilizumab (Actemra)
特异性（Specificity）：anti-tocilizumab antibodies
灵敏度（Sensitivity）：detection limit 1.5 ng/mL应用（Application）：measure anti-tocilizumab antibodies
检测类型（Method type）：Bridging ELISA
检测方法（Detection Method）：Peroxidase / OD450
检测精度（Assay precision）：Intra-assay coefficient of variation (CV) <10%. Inter-assay CV was <10%
酶标板类型（Plate）：Strip
样本类型（Sample type）：Serum and Plasma
样本体积（Sample volume）：15ul
防腐剂（Preservative）：None
试剂盒组分（Components）：
Coated microtiter plate, 96 wells
QC samples - 8x50ul
10X wash buffer - 50ml
Assay buffer - 50ml
1000X secondary antibody - 17ul
1000X detection reagent - 17ul
TMB - 12ml
TMB stop solution - 12ml
Plate sealers - 3
运输条件（Shipping）：Dry ice干冰
储存（Storage）：Stable at -20℃ for 1 year
托珠单抗免疫原性ELISA试剂盒，Tocilizumab (Actemra®) Immunogenicity ELISA Kit，专用于高特异性、高灵敏度检测血清和血浆中游离托珠单抗的定量测定。该药物是一种免疫抑制性人源化单克隆抗体，主要适用于类风湿性关节炎（RA）及全身型幼年特发性关节炎（一种严重的儿童关节炎）的治疗。
背景信息（Background information）：
Tocilizumab (Actemra® ) is a humanized recombinant monoclonal antibody used for the treatment of inflammatory types of arthritis, such as rheumatoid arthritis (RA). Tocilizumab works by binding and blocking the activity of the IL6 receptor. This kit allows researchers to measure anti-tocilizumab antibodies in biological matrices.
检测原理（Summary of the assay）：
This immunogenicity assay employs the bridging ELISA technique. Capture antibody (Tocilizumab) is precoated onto a 96 well microplate. Quality control and test samples are pipetted into the appropriate wells. Anti-tocilizumab present in biological matrices is bound by the immobilized capture antibody. After washing away any unbound substances, secondary antibody is added to the wells and after a final wash a detection reagent is added. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of anti-tocilizumab present in test samples. QC samples give a quantitative reference signal which can be used to determine the level of anti-tocilizumab antibody in the unknown samples. The color development is stopped and the intensity of the color is measured.
样本制备（Sample preparation）：
[注意事项]：勃谱生物的托珠单抗免疫原性ELISA试剂盒(Tocilizumab ELISA试剂盒)产品仅用于研究用途。不可用于诊断、医疗或其他用途。
[NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.
返回列表
特异性（Specificity）：anti-tocilizumab antibodies
灵敏度（Sensitivity）：detection limit 1.5 ng/mL应用（Application）：measure anti-tocilizumab antibodies
检测类型（Method type）：Bridging ELISA
检测方法（Detection Method）：Peroxidase / OD450
检测精度（Assay precision）：Intra-assay coefficient of variation (CV) <10%. Inter-assay CV was <10%
酶标板类型（Plate）：Strip
样本类型（Sample type）：Serum and Plasma
样本体积（Sample volume）：15ul
防腐剂（Preservative）：None
试剂盒组分（Components）：
Coated microtiter plate, 96 wells
QC samples - 8x50ul
10X wash buffer - 50ml
Assay buffer - 50ml
1000X secondary antibody - 17ul
1000X detection reagent - 17ul
TMB - 12ml
TMB stop solution - 12ml
Plate sealers - 3
运输条件（Shipping）：Dry ice干冰
储存（Storage）：Stable at -20℃ for 1 year
托珠单抗免疫原性ELISA试剂盒，Tocilizumab (Actemra®) Immunogenicity ELISA Kit，专用于高特异性、高灵敏度检测血清和血浆中游离托珠单抗的定量测定。该药物是一种免疫抑制性人源化单克隆抗体，主要适用于类风湿性关节炎（RA）及全身型幼年特发性关节炎（一种严重的儿童关节炎）的治疗。
背景信息（Background information）：
Tocilizumab (Actemra® ) is a humanized recombinant monoclonal antibody used for the treatment of inflammatory types of arthritis, such as rheumatoid arthritis (RA). Tocilizumab works by binding and blocking the activity of the IL6 receptor. This kit allows researchers to measure anti-tocilizumab antibodies in biological matrices.
检测原理（Summary of the assay）：
This immunogenicity assay employs the bridging ELISA technique. Capture antibody (Tocilizumab) is precoated onto a 96 well microplate. Quality control and test samples are pipetted into the appropriate wells. Anti-tocilizumab present in biological matrices is bound by the immobilized capture antibody. After washing away any unbound substances, secondary antibody is added to the wells and after a final wash a detection reagent is added. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of anti-tocilizumab present in test samples. QC samples give a quantitative reference signal which can be used to determine the level of anti-tocilizumab antibody in the unknown samples. The color development is stopped and the intensity of the color is measured.
样本制备（Sample preparation）：
[注意事项]：勃谱生物的托珠单抗免疫原性ELISA试剂盒(Tocilizumab ELISA试剂盒)产品仅用于研究用途。不可用于诊断、医疗或其他用途。
[NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.
