SARS-CoV-2替代病毒中和试验（sVNT）试剂盒
货号：EL-1611-32214
品牌：AffinityImmuno
产品特性
SARS-CoV-2 surrogate Virus Neutralizing Test (sVNT) Kit
Reactivity：human
Application：Quantification of neutralizing antibodies
Methode type：Competitive ELISA
Sample type：Serum and Plasma
| 规格 | 价格 |
|---|---|
| 96 wells | ￥咨询 |
| 3 x 96 wells | ￥咨询 |
| 5 x 96 wells | ￥咨询 |
产品咨询：info@biopcr.com
产品订购：sales@biopcr.com
技术支持：tech@biopcr.com
服务热线：400-860-6200
详细介绍
产品名称：SARS-CoV-2替代病毒中和试验（sVNT）试剂盒
别名：SARS-CoV-2 surrogate Virus Neutralizing Test (sVNT) (spike protein/ ACE2 ligand binding assay) COVID-19 sVNT；SARS-CoV-2替代病毒中和试验（sVNT）（刺突蛋白/ACE2配体结合检测法）；COVID-19 sVNT 试剂盒；SARS-CoV-2 sVNT试剂盒；sVNT中和抗体检测试剂盒；SARS-CoV-2假病毒中和试验试剂盒；新型冠状病毒中和抗体ELISA试剂盒；SARS-CoV-2替代病毒中和试验试剂盒
抗原（Antigen）：Neutralizing antibodies
反应性（Reactivity）：human
特异性（Specificity）：SARS-CoV-2 neutralizing antibodies
应用（Application）：Quantification of neutralizing antibodies
检测类型（Method type）：Competitive ELISA
检测方法（Detection Method）：Peroxidase / OD450
检测精度（Assay precision）：Intra-assay coefficient of variation (CV) <10%. Inter-assay CV was <10%
酶标板类型（Plate）：Strip
样本类型（Sample type）：Serum and Plasma
样本体积（Sample volume）：40 uL
检测时间（Assay time）：2.5 hours
防腐剂（Preservative）：none
试剂盒组分（Components）：
Coated microtiter plate, 96 wells
Calibrators (250 uL) - 0%, 20%, 40%, 60%, 80%, 100% inhibition
10X wash buffer - 50ml
Assay buffer - 50ml
1000X detection reagent - 150 ul
TMB - 12ml
TMB stop solution - 12ml
Plate sealers - 3
运输条件（Shipping）：Wet ice湿冰
储存（Storage）：Stable at -20℃ for 1 year
SARS-CoV-2替代病毒中和检测试剂盒，SARS-CoV-2 surrogate Virus Neutralizing Test (sVNT) Kit，本SARS-CoV-2/COVID-19 sVNT试剂盒可通过与中和抗体结合，快速检测潜在的COVID-19免疫反应。
背景信息（Background information）：
Neutralizing antibodies develop in some patients to varying degrees following infection with SARS-CoV-2
检测原理（Summary of the assay）：
SARS-CoV-2 spike protein ligand binding assay allows rapid quantification of COIVD19 neutralizing antibodies in serum that block the interaction between the viral spike protein and its target the ACE2 receptor.
样本采集（Sample collection）：
This kit is compatible with EDTA-plasma, heparinplasma and serum samples. Samples can be stored at or below -20°C for up to 1 year.
样本制备（Sample preparation）：
Samples are used neat.
试剂准备（Reagent preparation）：
Prepare only the appropriate amount of required reagent on the day of use. Store all reagents as per instructions stated on the label.
检测程序（Assay procedure）：
This is a competitive ELISA that measures COVID19 neutralizing antibodies in human sera and plasma that prevents the binding of viral spike protein to its ACE2 receptor target.
结果计算（Results calculation）：
1. Neutralizing antibodies will vary in terms of concentration and affinity, this assay provides a qualitative readout. As such the user should use the comparable positive controls inhibitory controls when comparing interassay results. The provided controls are tested for comparability between lots and can be traced.
2. The protein titers in the test samples will fall in the range of high, medium, low or negative. We recommend each lab develop their own statistical cutpoint using methodologies as described by G. Shankar, et al. (2008). (Recommendations for the validation of immunoassays used for detection of host antibodies against biotechnology products. J. Pharmaceutical and Biomedical Analysis 48:1267–1281).
3. Any sample undiluted or diluted still reading greater than the highest standard should be diluted appropriately with assay buffer and retested. If the samples have been diluted, the concentration determined from the standard curve must be multiplied by the dilution factor.
[注意事项]：勃谱生物的SARS-CoV-2替代病毒中和检测试剂盒(SARS-CoV-2 sVNT Kit)产品仅用于研究用途。不可用于诊断、医疗或其他用途。
[NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.
返回列表
反应性（Reactivity）：human
特异性（Specificity）：SARS-CoV-2 neutralizing antibodies
应用（Application）：Quantification of neutralizing antibodies
检测类型（Method type）：Competitive ELISA
检测方法（Detection Method）：Peroxidase / OD450
检测精度（Assay precision）：Intra-assay coefficient of variation (CV) <10%. Inter-assay CV was <10%
酶标板类型（Plate）：Strip
样本类型（Sample type）：Serum and Plasma
样本体积（Sample volume）：40 uL
检测时间（Assay time）：2.5 hours
防腐剂（Preservative）：none
试剂盒组分（Components）：
Coated microtiter plate, 96 wells
Calibrators (250 uL) - 0%, 20%, 40%, 60%, 80%, 100% inhibition
10X wash buffer - 50ml
Assay buffer - 50ml
1000X detection reagent - 150 ul
TMB - 12ml
TMB stop solution - 12ml
Plate sealers - 3
运输条件（Shipping）：Wet ice湿冰
储存（Storage）：Stable at -20℃ for 1 year
SARS-CoV-2替代病毒中和检测试剂盒，SARS-CoV-2 surrogate Virus Neutralizing Test (sVNT) Kit，本SARS-CoV-2/COVID-19 sVNT试剂盒可通过与中和抗体结合，快速检测潜在的COVID-19免疫反应。
背景信息（Background information）：
Neutralizing antibodies develop in some patients to varying degrees following infection with SARS-CoV-2
检测原理（Summary of the assay）：
SARS-CoV-2 spike protein ligand binding assay allows rapid quantification of COIVD19 neutralizing antibodies in serum that block the interaction between the viral spike protein and its target the ACE2 receptor.
样本采集（Sample collection）：
This kit is compatible with EDTA-plasma, heparinplasma and serum samples. Samples can be stored at or below -20°C for up to 1 year.
样本制备（Sample preparation）：
Samples are used neat.
试剂准备（Reagent preparation）：
Prepare only the appropriate amount of required reagent on the day of use. Store all reagents as per instructions stated on the label.
检测程序（Assay procedure）：
This is a competitive ELISA that measures COVID19 neutralizing antibodies in human sera and plasma that prevents the binding of viral spike protein to its ACE2 receptor target.
结果计算（Results calculation）：
1. Neutralizing antibodies will vary in terms of concentration and affinity, this assay provides a qualitative readout. As such the user should use the comparable positive controls inhibitory controls when comparing interassay results. The provided controls are tested for comparability between lots and can be traced.
2. The protein titers in the test samples will fall in the range of high, medium, low or negative. We recommend each lab develop their own statistical cutpoint using methodologies as described by G. Shankar, et al. (2008). (Recommendations for the validation of immunoassays used for detection of host antibodies against biotechnology products. J. Pharmaceutical and Biomedical Analysis 48:1267–1281).
3. Any sample undiluted or diluted still reading greater than the highest standard should be diluted appropriately with assay buffer and retested. If the samples have been diluted, the concentration determined from the standard curve must be multiplied by the dilution factor.
[注意事项]：勃谱生物的SARS-CoV-2替代病毒中和检测试剂盒(SARS-CoV-2 sVNT Kit)产品仅用于研究用途。不可用于诊断、医疗或其他用途。
[NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.
