产品特性
5-HT (Serotonin) 7 Receptor Antibody
应用：ICC,IF,IHC,WB验证
Host:Rabbit；Immunogen：Rat 5-HT7 receptor (8-23)
高文献引用数据支持（通过CiteAb官网可查）
| 规格 | 价格 |
|---|---|
| 100 µL | ￥咨询 |
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详细介绍
产品名称：5-HT (Serotonin) 7受体抗体
别名：5-HT7受体抗体；5-羟色胺7受体抗体；5-HT7 Receptor Antibody；5-hydroxytryptamine receptor 7; 5-HT-X,GPRFO; 5Ht7; 5-hydroxytryptamine (serotonin) receptor 7, anti-5-HT 7
宿主：Rabbit
应用：ICC,IF,IHC,WB验证
免疫原：Rat 5-HT7 receptor (8-23)
反应性：Cat, Grasshopper, Human, Hutchinsoniella Macracantha (Horseshoe Shrimp), Mouse, Pig, Rabbit, Rat
形式：液体
描述：
The ImmunoStar 5-HT7 receptor antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat cortex and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/100 – 1/300 in PBS – biotin/avidin-HRP Technique. Note: use of Triton X-100 or other detergents is not recommended.
The antibody was characterized by immunohistochemistry. Immunohistochemical staining of rat brain correlates well with Northern blot analysis, in situ hybridization and receptor autoradiography studies.Due to the difficulty with receptor antibodies, blot applications are not warranted and are included as specificity information only.
Immunolabeling is completely abolished by preadsorption with synthetic rat 5-HT7 receptor (8-23). BlastP database sequence homology searches indicate that the amino acid sequence is unique to rat 5-HT7A, 5-HT7B and 5-HT7C. There is also significant sequence overlap with the mouse and human forms.
图片说明：
IHC image of neurons staining for the 5-HT7 receptor in the rat brain cortex. The tissue was fixed with 4% formaldehyde/0.05% glutaraldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:500 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
RRID：AB_572214
GeneSymbol：Htr7
运输条件：Blue ice
返回列表
The ImmunoStar 5-HT7 receptor antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat cortex and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/100 – 1/300 in PBS – biotin/avidin-HRP Technique. Note: use of Triton X-100 or other detergents is not recommended.
The antibody was characterized by immunohistochemistry. Immunohistochemical staining of rat brain correlates well with Northern blot analysis, in situ hybridization and receptor autoradiography studies.Due to the difficulty with receptor antibodies, blot applications are not warranted and are included as specificity information only.
Immunolabeling is completely abolished by preadsorption with synthetic rat 5-HT7 receptor (8-23). BlastP database sequence homology searches indicate that the amino acid sequence is unique to rat 5-HT7A, 5-HT7B and 5-HT7C. There is also significant sequence overlap with the mouse and human forms.
图片说明：
IHC image of neurons staining for the 5-HT7 receptor in the rat brain cortex. The tissue was fixed with 4% formaldehyde/0.05% glutaraldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:500 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
RRID：AB_572214
GeneSymbol：Htr7
运输条件：Blue ice
