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5-HT (Serotonin)山羊抗体

货号:20079-100ul 品牌:ImmunoStar

产品特性

5-HT (Serotonin) Goat Antibody
应用:ICC,IF,IHC,WB验证
Host:Goat;Immunogen:Serotonin
高文献引用数据支持(通过CiteAb官网可查)
规格 价格
100 µL 咨询
产品咨询:info@biopcr.com
产品订购:sales@biopcr.com
技术支持:tech@biopcr.com
服务热线:400-860-6200

详细介绍

产品名称:5-HT (Serotonin)山羊抗体
别名:anti-5-HT Goat;5-HT Goat Antibody;5-HT抗体;山羊5-羟色胺抗体
宿主:Goat
应用:ICC,IF,IHC,WB验证
免疫原:Serotonin
反应性:Cat, Cod, Cow, Crayfish, Dolphin, Fish, Frog (Xenopus Laevis), Guinea Pig, Hamster, Human, Mexican salamander (Ambystoma Mexicanum), Monkey, Moth, Mouse, Pig, Rabbit, Ram (Sheep), Rat, Salamander, Snail, Tapeworm, Worm, Zebrafish
形式:Lyophilized Whole Serum
描述:
The ImmunoStar serotonin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/5000 – 1/10,000 in PBS/0.3% Triton X-100 – Bn/Av-HRP Technique.

Staining is completely eliminated by pretreatment of the diluted antibody with 100 µg of serotonin/BSA conjugate.

图片说明:
Low magnification of IHC image of neurons staining for the 5-HT goat antibody in the pontine region of the rat brain (top), and high magnification of the raphe nucleus (bottom). The tissue was fixed with 4% formaldehyde/0.05% glutaraldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% rabbit serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:7500 for 48 hours, rabbit anti-goat secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.

RRID:AB_572262
GeneSymbol:Spl
运输条件:Blue ice
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