产品特性
Calbindin D-28K Antibody
应用:ICC,IF,IHC,WB验证
Host:Rabbit;Immunogen:Bovine Calbindin D-28K
高文献引用数据支持(通过CiteAb官网可查)
规格 | 价格 |
---|---|
100 µL | ¥咨询 |
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详细介绍
产品名称:Calbindin D-28K抗体
别名:钙结合蛋白D-28K抗体;Calbindin; nCAB27; Calbindin D28; D-28K; Vitamin D-dependent calcium-binding protein; Vitamin D-dependent calcium-binding protein, avian-type; CALB1; calbindin 1, 28kDa, anti-Calbindin
宿主:Rabbit
应用:ICC,IF,IHC,WB验证
免疫原:Bovine Calbindin D-28K
反应性:Mouse, Rat
形式:Lyophilized Whole Serum
描述:
The antibody has a proven maximum biotin-streptavidin/HRP staining at a 1/5,000 – 1/10,000 dilution in rat striatum, cortex, and hippocampus.
The antiserum has been characterized as specific to calbindin D-28k; please see reference listed below. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended.
图片说明:
IHC image of neurons staining for the calbindin in the rat amygdala (above) and the rat cortex (below). The tissue was fixed with 4% formaldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:10000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. The section was then mounted on slides with permount.
RRID:AB_572222
GeneSymbol:CALB1
运输条件:Blue ice
返回列表
The antibody has a proven maximum biotin-streptavidin/HRP staining at a 1/5,000 – 1/10,000 dilution in rat striatum, cortex, and hippocampus.
The antiserum has been characterized as specific to calbindin D-28k; please see reference listed below. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended.
图片说明:
IHC image of neurons staining for the calbindin in the rat amygdala (above) and the rat cortex (below). The tissue was fixed with 4% formaldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:10000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. The section was then mounted on slides with permount.
RRID:AB_572222
GeneSymbol:CALB1
运输条件:Blue ice