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CGRP (Calcitonin Gene Related Peptide)抗体

货号:24112-100ul 品牌:ImmunoStar

产品特性

CGRP (Calcitonin Gene Related Peptide) Antibody
应用:ICC,IF,IHC,WB验证
Host:Rabbit;Immunogen:rat alpha-CGRP
高文献引用数据支持(通过CiteAb官网可查)
规格 价格
100 µL 咨询
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详细介绍

产品名称:CGRP (Calcitonin Gene Related Peptide)抗体
别名:anti-CGRP;Calcitonin Gene Related Peptide Antibody;降钙素基因相关肽抗体
宿主:Rabbit
应用:ICC,IF,IHC,WB验证
免疫原:rat alpha-CGRP
反应性:Bullfrog, Cat, Dog, Hamster, Human, Mouse, Mudpuppy (Necturus Maculosus), Ovine (Sheep), Pig, Platypus, Ram (Sheep), Rat
形式:Lyophilized Whole Serum
描述:
The antibody has a proven and strong Biotin-Streptadvidin/HRP staining at a 1/2000-1/4000 dilution in rat amygdala, and spinal cord.

The specificity of the antiserum was evaluated by soluble pre-adsorption with the peptides in question at a final concentration of 10-5M. CGRP immunolabeling was completely abolished by pre-adsorption with rat α -CGRP and partially eliminated by pre-adsorption with rat Ÿ -CGRP. Pre-adsorption with the following peptides resulted in no loss of immunostaining: rat amylin, rat adrenomedulin, calcitonin, neurotensin, somatostatin, substance P, leucine enkephalin, methionine enkephalin, VIP, CCK-8, vasopressin and neuropeptide Y.

图片说明:
IHC image of the dorsal horn of the rat spinal cord staining for CGRP (above) and low magnification image of a coronal spinal cord section staining for CGRP (below). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for frozen sectioning. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:4000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.

RRID:AB_572217
GeneSymbol:Calca
运输条件:Blue ice
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