钙(Ca)检测试剂盒(Chlorophosphonazo III)
货号：CCA-020
品牌：JaICA
产品特性
Quick & Easy to use.
Species independent!
No toxic substance!
Highly sensitive, stable and suitable for high-throughput testing!
Applicable to cell lysates, serum, plasma, urine, saliva, extracts from tissues, plants, hair and bone samples.
| 规格 | 价格 |
|---|---|
| 200 tests | ￥咨询 |
产品咨询：info@biopcr.com
产品订购：sales@biopcr.com
技术支持：tech@biopcr.com
服务热线：400-860-6200
详细介绍
产品名称：钙(Ca)检测试剂盒(Chlorophosphonazo III)
别名：Calcium Colorimetric Assay Kit（Chlorophosphonazo III）；Calcium Assay Kit；Ca Assay Kit；钙比色法检测试剂盒；钙含量检测试剂盒；钙检测试剂盒
应用：Colorimetric: 680-700 nm
特异性：Calcium (Ca)
检测方法：Chlorophosponazo-III Chromogenic method
检测范围：0.2-20 mg/dL
储存条件：2 - 8°C (避免冷冻).
产品描述：
Calcium is the most abundant and one of the most important minerals in the human body. Approximately 99% of body calcium is found in bones. The calcium level in the extracellular space is in dynamic equilibrium with bone calcium. Calcium ions affect the contractility of heart and skeletal muscles and are essential for the function of the nervous system. Calcium ions play an important role in blood clotting and bone minilization. In plasma, calcium is bound to a considerable extent to proteins, 10 % is in the form of inorganic complexes and 50 % is present as free ion species. The calcium homeostasis regulated by the parathyroid hormone (PTH), calcitriol (CT), and calcitonin. A decrease in albumin level causes a decrease in serum calcium. Low levels of calcium are found in hypoparathyroidism, pseudohypoparathyroidism, vitamin D deficiency, malnutrition and intestinal malabsortion. Among causes of hypercalcemia are cancers, large intake of vitamin D, enhanced renal retention, osteoporosis, sarcosidosis, thyrotoxicosis, hyperparathyroidism.
检测原理：
This product is a direct colorimetric assay kit without deproteinization of the sample. Calcium with Chlorophosphonazo-III (as chelator) at neutral pH, yields a blue colored complex. The intensity of the colour formed is proportional to the calcium concentration in the sample.
样本处理：
Serum/ Plasma: Insoluble substances in serum and plasma samples should be removed by filtration or centrifugation. EDTA-plasma cannot be used.
Urine (24 hour pooled urine) Dilute the sample 1/2 in distilled water. Add 6M HCl to the diluted sample and adjust pH 2.0-3.0 (e.g. 5-10 μL of 6M HCl/1mL of lysate). Centrifuge at 6,000 rpm for 15 minutes. Collect the supernatant and use it for assay.
Biological fluid: Add 6M HCl to the sample and adjust pH 2.0-3.0 (e.g. 5-10 μL of 6M HCl/1mL of lysate). Centrifuge at 6,000 rpm for 15 minutes. Collect the supernatant and use it for assay.
Tissue: Add 5% TCA solution, voltex for 1 minute and incubate for 30 minutes at 4-8°C. Centrifuge at 6,000 rpm for 15 minutes. Collect the supernatant and use it for assay.
Note: Sample pH should be between pH 2 and pH 8.
试剂盒组分：
• R-1螯合显色液（即用型）：50 mL ×1瓶
• 钙标准品（10 mg/dL，即用型）：1.0 mL ×1瓶
[注意事项]：该钙比色法检测试剂盒（Chlorophosphonazo III）仅用于研究用途。不可用于诊断、医疗或其他用途。
[NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.
返回列表
Calcium is the most abundant and one of the most important minerals in the human body. Approximately 99% of body calcium is found in bones. The calcium level in the extracellular space is in dynamic equilibrium with bone calcium. Calcium ions affect the contractility of heart and skeletal muscles and are essential for the function of the nervous system. Calcium ions play an important role in blood clotting and bone minilization. In plasma, calcium is bound to a considerable extent to proteins, 10 % is in the form of inorganic complexes and 50 % is present as free ion species. The calcium homeostasis regulated by the parathyroid hormone (PTH), calcitriol (CT), and calcitonin. A decrease in albumin level causes a decrease in serum calcium. Low levels of calcium are found in hypoparathyroidism, pseudohypoparathyroidism, vitamin D deficiency, malnutrition and intestinal malabsortion. Among causes of hypercalcemia are cancers, large intake of vitamin D, enhanced renal retention, osteoporosis, sarcosidosis, thyrotoxicosis, hyperparathyroidism.
检测原理：
This product is a direct colorimetric assay kit without deproteinization of the sample. Calcium with Chlorophosphonazo-III (as chelator) at neutral pH, yields a blue colored complex. The intensity of the colour formed is proportional to the calcium concentration in the sample.
样本处理：
Serum/ Plasma: Insoluble substances in serum and plasma samples should be removed by filtration or centrifugation. EDTA-plasma cannot be used.
Urine (24 hour pooled urine) Dilute the sample 1/2 in distilled water. Add 6M HCl to the diluted sample and adjust pH 2.0-3.0 (e.g. 5-10 μL of 6M HCl/1mL of lysate). Centrifuge at 6,000 rpm for 15 minutes. Collect the supernatant and use it for assay.
Biological fluid: Add 6M HCl to the sample and adjust pH 2.0-3.0 (e.g. 5-10 μL of 6M HCl/1mL of lysate). Centrifuge at 6,000 rpm for 15 minutes. Collect the supernatant and use it for assay.
Tissue: Add 5% TCA solution, voltex for 1 minute and incubate for 30 minutes at 4-8°C. Centrifuge at 6,000 rpm for 15 minutes. Collect the supernatant and use it for assay.
Note: Sample pH should be between pH 2 and pH 8.
试剂盒组分：
• R-1螯合显色液（即用型）：50 mL ×1瓶
• 钙标准品（10 mg/dL，即用型）：1.0 mL ×1瓶
[注意事项]：该钙比色法检测试剂盒（Chlorophosphonazo III）仅用于研究用途。不可用于诊断、医疗或其他用途。
[NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.
