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锌(Zn)检测试剂盒(5-Br-PAPS)

货号:CZN-001 品牌:JaICA

产品特性

样本无需预处理
跨物种通用检测
不含任何有毒物质
适配微孔板检测系统
操作步骤简便. 适用于检测细胞裂解液、血清、血浆及多种生物样本。
规格 价格
100 tests 咨询
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详细介绍

产品名称:锌(Zn)检测试剂盒(5-Br-PAPS)
别名:Zinc Colorimetric Assay Kit;Zinc Assay Kit;Zn Assay Kit;锌比色法检测试剂盒;锌含量检测试剂盒;锌(Zn)检测试剂盒;锌检测试剂盒
应用:10 minutes at room temperature (25 - 37 °C)
特异性:Zinc (Zn 2+)
检测方法:5-Br-PAPS method (Colorimetric assay at 560 (550-580) nm)
样本类型:Serum, plasma, urine, saliva, cell lysate, tissue extract, plant extract, hair extract, food extract and water. Will be interfered by EDTA.
检测范围:4 - 1,000 µg/dL
灵敏度:O.D. at 200 µg/dL standard will be 0.1 - 0.3
精密度:Accuracy: ±15% of known concentration;Intra assay variation: C.V. is less than 5%
储存条件:2 - 8°C (避免冷冻).
产品优势:
◆ Pre-preatment free!
◆ Species independent!
◆ No toxic substance!
◆ Applicable to microplate reader!

产品描述:
Zinc is an essential mineral cofactor of more than 200 enzymes which involved in nucleic acid, and protein synthesis. In mammals, acute zinc deficiency during the growth period is reported to cause failure in skin and hair, and growth retardation. This kit is a simple colorimetric assay which can detect zinc precisely in biological fluids in a short period of time (about 10 minutes).

检测原理:
Protein-bonded zinc is dissociated by denaturing agent. Free and protein-bonded zinc are mixed with 5-Br-PAPS to form chelate complex. Zinc-5-Br-PAPS complex can be detected by measuring absorbance at 560 nm.

本检测法不受以下物质影响
1. 血红蛋白:高达0.5 g/dL
2. 胆红素:高达15毫克/分升
3. 甘油三酯:高达1000mg /dL
这个实验会被EDTA干扰。
※ 该检测会受到EDTA的干扰

试剂盒组分:
R-1 缓冲液(即用型):24 mL
R-2 螯合显色剂(即用型):0.5 mL
锌标准品 200 µg/dL(即用型):1.2 mL

需用户自备
• 蒸馏水
• 96孔微孔板
• 微量移液器及配套吸头
• 酶标仪(检测波长560 nm)

[注意事项]:该锌(Zn)比色法检测试剂盒((5-Br-PAPS)仅用于研究用途。不可用于诊断、医疗或其他用途。
[NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.
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