钙(Ca)检测试剂盒(OCPC)
货号：CCA-030
品牌：JaICA
产品特性
样本无需预处理
跨物种通用检测
不含任何有毒物质
高灵敏度、稳定性佳且适用于高通量检测
极易操作. 适用于检测细胞裂解液、血清、血浆及多种生物样本。适用于细胞裂解液、血清、血浆、尿液、唾液，以及组织、植物、毛发样本和骨骼样本的提取物。
| 规格 | 价格 |
|---|---|
| 200 tests | ￥咨询 |
产品咨询：info@biopcr.com
产品订购：sales@biopcr.com
技术支持：tech@biopcr.com
服务热线：400-860-6200
详细介绍
产品名称：钙(Ca)检测试剂盒(OCPC)
别名：Calcium Colorimetric Assay Kit；Calcium Assay Kit；Ca Assay Kit；钙比色法检测试剂盒；钙含量检测试剂盒；钙检测试剂盒
应用：10 minutes at room temperature (25 - 37 °C)
特异性：Calcium (Ca)
检测方法：o-Cresolphtalein-Complexone(OCPC) method (Colorimetric assay at 570 nm)
样本类型：Serum, plasma, urine, saliva, cell lysate, tissue extract, plant extract, hair extract, food extract and water. The effect of 15 mg / dL of magnesium is less than 10%. Will be affected by EDTA.
检测范围：0.2～30 mg/dL
灵敏度：O.D. at 10 mg/dL standard will be 0.5 - 1.0
精密度：Accuracy:±15% of known concentration; Intra assay variation:C.V. is less than 5%
储存条件：2 - 8°C (避免冷冻).
产品优势：
◆ Pre-preatment free!
◆ Species independent!
◆ No toxic substance!
◆ Applicable to microplate reader!
产品描述：
Calcium is the most abundant mineral which accounts for 1.5-2.9% of body weight, and is an important element in the formation of bone and cartilaginous tissues. In the human bodies, 90% of the calcium is present as hydroxyapatite in bone, 1% is in cells, and 0.1% is in the blood. Calcium is known to plays important roles in various physiological functions such as osmotic homeostasis, blood clotting, muscle atrophy, nerve stimulation, and the activation of enzymes. In blood, 45% of calcium is attached to albumin or globulin, 10% is present as phosphate salt, and 45% exist as free ion.
检测原理：
o-Cresolphthalein Complexone (OCPC) and calcium can form a chelate complex with deep red color. The concentration of calcium can be determined by the absorbance at 570 nm. Although most OCPC-based calcium assay has sigmoid-shaped calibration plot, the calibration plot of this kit is linear and the calcium concentration can be estimated by simple one-point calibration.
试剂盒组分：
R-1 Buffer (ready to use): 38 mL
R-2 Chelate color (ready to use): 10 mL
Calcium standard 10 mg/dL (ready to use): 1 mL
需用户自备
Distilled water
96 well microtiter plate
Micropipettors and pipette tips
Microtiter plate reader(measuring at 570 nm, and 540 or 650-700 nm as reference)
[注意事项]：该Calcium Assay Kit (OCPC)仅用于研究用途。不可用于诊断、医疗或其他用途。
[NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.
返回列表
◆ Pre-preatment free!
◆ Species independent!
◆ No toxic substance!
◆ Applicable to microplate reader!
产品描述：
Calcium is the most abundant mineral which accounts for 1.5-2.9% of body weight, and is an important element in the formation of bone and cartilaginous tissues. In the human bodies, 90% of the calcium is present as hydroxyapatite in bone, 1% is in cells, and 0.1% is in the blood. Calcium is known to plays important roles in various physiological functions such as osmotic homeostasis, blood clotting, muscle atrophy, nerve stimulation, and the activation of enzymes. In blood, 45% of calcium is attached to albumin or globulin, 10% is present as phosphate salt, and 45% exist as free ion.
检测原理：
o-Cresolphthalein Complexone (OCPC) and calcium can form a chelate complex with deep red color. The concentration of calcium can be determined by the absorbance at 570 nm. Although most OCPC-based calcium assay has sigmoid-shaped calibration plot, the calibration plot of this kit is linear and the calcium concentration can be estimated by simple one-point calibration.
试剂盒组分：
R-1 Buffer (ready to use): 38 mL
R-2 Chelate color (ready to use): 10 mL
Calcium standard 10 mg/dL (ready to use): 1 mL
需用户自备
Distilled water
96 well microtiter plate
Micropipettors and pipette tips
Microtiter plate reader(measuring at 570 nm, and 540 or 650-700 nm as reference)
[注意事项]：该Calcium Assay Kit (OCPC)仅用于研究用途。不可用于诊断、医疗或其他用途。
[NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.
