铜(Cu)检测试剂盒(3,5-DiBr-PAESA)
货号：CCU-400
品牌：JaICA
产品特性
样本无需预处理
可检测各种物种
不含任何有毒物质
适配微孔板检测系统
操作步骤简便. 适用于检测细胞裂解液、血清、血浆及多种生物样本。
| 规格 | 价格 |
|---|---|
| 100 tests | ￥咨询 |
产品咨询：info@biopcr.com
产品订购：sales@biopcr.com
技术支持：tech@biopcr.com
服务热线：400-860-6200
详细介绍
产品名称：铜(Cu)检测试剂盒(3,5-DiBr-PAESA)
别名：Copper Assay Kit；Copper Colorimetric Assay Kit；Cu Assay Kit；铜含量检测试剂盒；铜比色法检测试剂盒；铜检测试剂盒
应用：10 minutes
特异性：Specific to Cu2+ and Cu+
检测方法：DiBr-PAESA method (Colorimetric assay at 580 (570-590) nm)
样本类型：Serum, plasma, urine, saliva, cell lysate, tissue extract, plant extract, hair extract, food extract and water. Will be interfered by EDTA.
检测范围：3 - 400 µg/dL
灵敏度：O.D. at 200 µg/dL standard will be 0.03 - 0.1
精密度：Accuracy: ±10% of known concentration；Intra assay variation: C.V. is less than 5%
储存条件：2 - 8°C (避免冷冻).
产品优势：
◆ Pre-preatment free!
◆ Species independent!
◆ No toxic substance!
◆ Applicable to microplate reader!
产品描述：
Inside human body, approximately 95% of copper in the plasma is attached to alpha-2- globulin, ceruloplasmin and some enzymes with ferroxidase activity. The main role of protein containing copper is oxidation-reduction function. Copper deficiency is known to be associated with some diseases such as heart disease, osteoporosis, osteoarthritis, Menkes syndrome and Wilson's disease. It has been also reported that copper deficiency may cause reduction of anti-oxidative function in vivo. On the other hand, the excess copper is toxic. This kit is a simple colorimetric assay which can detect copper precisely in biological fluids in a short period of time (about 10 minutes).
检测原理：
In this assay system, copper is dissociated from ceruloplasmin and other copper containing proteins, by denaturing agent in acidic condition. Free and protein-associated copper will form a copper -3-5 DiBr-PAESA complex, which can be detected by absorbance at 580 nm.
试剂盒组分：
R-1 缓冲液（即用型）：24 mL
R-2 螯合显色剂（即用型）：0.5 mL
铜标准品 200 µg/dL（即用型）：1.2 mL
需用户自备
• 蒸馏水
• 96孔微孔板
• 微量移液器及配套吸头
• 酶标仪（检测波长580 nm）
[注意事项]：该铜(Cu)比色法检测试剂盒（3,5-DiBr-PAESA）仅用于研究用途。不可用于诊断、医疗或其他用途。
[NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.
返回列表
◆ Pre-preatment free!
◆ Species independent!
◆ No toxic substance!
◆ Applicable to microplate reader!
产品描述：
Inside human body, approximately 95% of copper in the plasma is attached to alpha-2- globulin, ceruloplasmin and some enzymes with ferroxidase activity. The main role of protein containing copper is oxidation-reduction function. Copper deficiency is known to be associated with some diseases such as heart disease, osteoporosis, osteoarthritis, Menkes syndrome and Wilson's disease. It has been also reported that copper deficiency may cause reduction of anti-oxidative function in vivo. On the other hand, the excess copper is toxic. This kit is a simple colorimetric assay which can detect copper precisely in biological fluids in a short period of time (about 10 minutes).
检测原理：
In this assay system, copper is dissociated from ceruloplasmin and other copper containing proteins, by denaturing agent in acidic condition. Free and protein-associated copper will form a copper -3-5 DiBr-PAESA complex, which can be detected by absorbance at 580 nm.
试剂盒组分：
R-1 缓冲液（即用型）：24 mL
R-2 螯合显色剂（即用型）：0.5 mL
铜标准品 200 µg/dL（即用型）：1.2 mL
需用户自备
• 蒸馏水
• 96孔微孔板
• 微量移液器及配套吸头
• 酶标仪（检测波长580 nm）
[注意事项]：该铜(Cu)比色法检测试剂盒（3,5-DiBr-PAESA）仅用于研究用途。不可用于诊断、医疗或其他用途。
[NOTICE]: Our products are for RESEARCH USE ONLY. Not for diagnostic, medical or other use.
